Anti-NFX1 antibody (ab176733)
Key features and details
- Rabbit polyclonal to NFX1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NFX1 antibody
See all NFX1 primary antibodies -
Description
Rabbit polyclonal to NFX1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human NFX1 aa 1070-1120. The exact sequence is proprietary. NP_002495.2.
Database link: Q12986 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NFX1 antibody (ab176733)All lanes : Anti-NFX1 antibody (ab176733) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-NFX1 antibody (ab176733)ab176733 at 0.4 µg/ml detecting NFX1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of NFX1.
Lane 2: IP with an antibody which recognizes an upstream epitope of NFX1.Lane 3: ab176733 at 3 µg/mg of lysate
Lane 4: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
