Antibodies, Proteins, Kits and Reagents for Life Science

Anti-NFkB p100/NFKB2 antibody (ab264236)

Key features and details

Rabbit polyclonal to NFkB p100/NFKB2
Suitable for: IP, WB
Reacts with: Mouse, Human
Isotype: IgG

Product name

Anti-NFkB p100/NFKB2 antibody
See all NFkB p100/NFKB2 primary antibodies
Description

Rabbit polyclonal to NFkB p100/NFKB2
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human NFkB p100/NFKB2 aa 810-860. The exact sequence is proprietary. NP_001070962.1
Database link: Q00653
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Intracellular
- NFkB
- p52
- Cancer
- Cell Death
- Apoptosis
- Apoptosis Markers
- NFkB
- p52

Western blot - Anti-NFkB p100/NFKB2 antibody (ab264236)

All lanes : Anti-NFkB p100/NFKB2 antibody (ab264236) at 0.1 µg/ml

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 50 µg per lane.

Developed using the ECL technique.

Predicted band size: 97 kDa

Exposure time: 3 minutes

Lysates prepared in NETN buffer.
Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody (ab264236)

NFkB p100 / p52 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1.0
mg per IP reaction; 20% of IP loaded) using ab264236 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264236 at 1 μg/ml.

Lane 1: ab264236 IP in HeLa whole cell lysate.

Lane 2: Control IgG IP in Hela whole cell lysate.

Exposure time: 3 minutes.

Lysates prepared in NETN buffer.

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