Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Netrin 1 antibody [EPR5428] (ab126729)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5428] to Netrin 1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Netrin 1 antibody [EPR5428]
    See all Netrin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5428] to Netrin 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Netrin 1 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: O95631
    (Peptide available as ab192272)

  • Positive control

    • WB: Human fetal brain, human small intestine, mouse kidney, mouse heart, rat kidney and brain tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    All lanes : Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/500 dilution

    Lane 1 : Rat brain lysates prepared in RIPA lysis method
    Lane 2 : Rat brain lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Developed using the ECL technique.

    Predicted band size: 67 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    All lanes : Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/1000 dilution (purified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/1000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 67 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    Western blot - Anti-Netrin 1 antibody [EPR5428] (ab126729)
    All lanes : Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human small intestine lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 67 kDa

  • Anti-Netrin 1 antibody [EPR5428] (ab126729)
    Anti-Netrin 1 antibody [EPR5428] (ab126729)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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