Anti-NEDD1 antibody (ab230254)
Key features and details
- Rabbit polyclonal to NEDD1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NEDD1 antibody
See all NEDD1 primary antibodies -
Description
Rabbit polyclonal to NEDD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human NEDD1 aa 400 to the C-terminus.
Database link: Q8NHV4 -
Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NEDD1 antibody (ab230254)All lanes : Anti-NEDD1 antibody (ab230254) at 1/1000 dilution
Lane 1 : Mouse small intestine tissue lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 72 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (ab230254)Paraffin-embedded human glioma cancer tissue stained for NEDD1 using ab230254 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (ab230254)Paraffin-embedded human thyroid tissue stained for NEDD1 using ab230254 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-NEDD1 antibody (ab230254)NEDD1 was immunoprecipitated from 0.5 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg ab230254. Western blot was performed from the immunoprecipitate using ab230254. An HRP-conjugated light chain specific antibody was used as secondary antibody at 1/50,000 dilution.
Lane 1: Rabbit monoclonal IgG (1 μg) instead of ab230254 in HeLa whole cell lysate.
Lane 2: ab230254 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
