Anti-NEDD1 antibody (ab230201)
Key features and details
- Rabbit polyclonal to NEDD1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NEDD1 antibody
See all NEDD1 primary antibodies -
Description
Rabbit polyclonal to NEDD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human NEDD1 aa 400 to the C-terminus.
Database link: Q8NHV4 -
Positive control
- WB: 293T, Jurkat, HepG-2, A549 and PC-3 cell lysate. IHC-P: Human glioma cancer tissue. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NEDD1 antibody (ab230201)All lanes : Anti-NEDD1 antibody (ab230201) at 1/2000 dilution
Lane 1 : Mouse liver tissue
Lane 2 : Mouse lung tissue
Lane 3 : Mouse kidney tissue
Lane 4 : 293T cell lysate
Lane 5 : Hela cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : HepG-2 cell lysate
Lane 8 : A549 cell lysate
Lane 9 : PC-3 cell lysate
Predicted band size: 72 kDaObserved MW: 72kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (ab230201)Paraffin-embedded human glioma cancer tissue stained for NEDD1 using ab230201 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-NEDD1 antibody (ab230201)NEDD1 was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using 4 µg of ab230201.
Lane 1: 1 µg Rabbit monoclonal IgG instead of ab230201 in HeLa whole cell lysate.
Lane 2: ab230201 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
