Anti-NBR1 antibody - C-terminal (ab226360)
Key features and details
- Rabbit polyclonal to NBR1 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NBR1 antibody - C-terminal
See all NBR1 primary antibodies -
Description
Rabbit polyclonal to NBR1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human NBR1 aa 916-966 (C terminal). The exact sequence is proprietary.
Database link: Q14596 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226360 was affinity purified using an epitope specific to NBR1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NBR1 antibody - C-terminal (ab226360)All lanes : Anti-NBR1 antibody - C-terminal (ab226360) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 107 kDa
Exposure time: 30 secondsCell lysates were prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-NBR1 antibody - C-terminal (ab226360)NBR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226360 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226360 at 1 µg/ml.
Lane 1: ab226360 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
