Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NAP1L1 antibody [EPR11845] (ab178687)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11845] to NAP1L1
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NAP1L1 antibody [EPR11845]
    See all NAP1L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11845] to NAP1L1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAP1L1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P55209

  • Positive control

    • HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899). Human kidney tissue. HeLa cells. Permeabilized 293T cells. Immunoprecipitation pellet from 293T cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NAP1L1 antibody [EPR11845] (ab178687)
    Western blot - Anti-NAP1L1 antibody [EPR11845] (ab178687)
    All lanes : Anti-NAP1L1 antibody [EPR11845] (ab178687) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293T lysate
    Lane 3 : A431 lysate
    Lane 4 : Jurkat lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] (ab178687)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] (ab178687)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with ab178687 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] (ab178687)
    Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] (ab178687)

    Immunofluorescence analysis of HeLa cells labeling NAP1L1 with ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-NAP1L1 antibody [EPR11845] (ab178687)
    Flow Cytometry - Anti-NAP1L1 antibody [EPR11845] (ab178687)

    Flow cytometric analysis of permeabilized 293T cells using ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] (ab178687)
    Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] (ab178687)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-NAP1L1 antibody [EPR11845] (ab178687)
    Anti-NAP1L1 antibody [EPR11845] (ab178687)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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