Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 09, 2021

Anti-NAP1L1 antibody [EPR11845] (ab178687)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11845] to NAP1L1
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP
Reacts with: Human

Product name

Anti-NAP1L1 antibody [EPR11845]
See all NAP1L1 primary antibodies
Description

Rabbit monoclonal [EPR11845] to NAP1L1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAP1L1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P55209
Positive control
- HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899). Human kidney tissue. HeLa cells. Permeabilized 293T cells. Immunoprecipitation pellet from 293T cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11845
Isotype

IgG
Research areas

Western blot - Anti-NAP1L1 antibody [EPR11845] (ab178687)

All lanes : Anti-NAP1L1 antibody [EPR11845] (ab178687) at 1/10000 dilution

Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : A431 lysate
Lane 4 : Jurkat lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] (ab178687)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with ab178687 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NAP1L1 antibody [EPR11845] (ab178687)

Immunofluorescence analysis of HeLa cells labeling NAP1L1 with ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).
Flow Cytometry - Anti-NAP1L1 antibody [EPR11845] (ab178687)

Flow cytometric analysis of permeabilized 293T cells using ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] (ab178687)

Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-NAP1L1 antibody [EPR11845] (ab178687)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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