Anti-Myc tag antibody [Myc.A7] (ab18185)
![Anti-Myc tag antibody [Myc.A7] (ab18185)](https://www.abcam.com/ps/products/18/ab18185/Images/ab18185-349614-Ab18185KOWB.jpg "Anti-Myc tag antibody [Myc.A7] (ab18185)")
Key features and details
- Mouse monoclonal [Myc.A7] to Myc tag
- Suitable for: ELISA, Dot blot, WB, IP
- Knockout validated
- Isotype: IgG1
Overview
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Product name
Anti-Myc tag antibody [Myc.A7]
See all Myc tag primary antibodies -
Description
Mouse monoclonal [Myc.A7] to Myc tag -
Host species
Mouse -
Specificity
Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells. -
Tested applications
Suitable for: ELISA, Dot blot, WB, IPmore details -
Immunogen
Synthetic peptide:
EQKLISEEDL
, corresponding to amino acids 410-419 of Human Myc. -
Positive control
- WB: HEK-293 whole cell lysate ICC/IF: rat primary hippocampal neurons
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General notes
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A affinity chromatography -
Clonality
Monoclonal -
Clone number
Myc.A7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T whole cell lysate
Lane 2 : MYC knockout HEK-293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab18185 observed at 48 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab18185 was shown to recognize MYC in wild-type HEK-293T cells as signal was lost at the expected MW in MYC knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MYC knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab18185 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)](https://www.abcam.com/ps/products/18/ab18185/Images/ab18185-274543-anti-myc-tag-antibody-myca7-western-blot.jpg)
Western blot - Anti-Myc tag antibody [Myc.A7] (ab18185)All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution
Lane 1 : HEK293 lysate - untransfected
Lane 2 : HEK293 lysate - transfected with Myc-tagged protein
