Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19967] to MX1 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-MX1 antibody [EPR19967] - BSA and Azide free
    See all MX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19967] to MX1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi treated with 5000U/ml IFN alpha for 48 hours whole cell lysate; Human spleen and tonsil lysates. ICC/IF: Daudi cells treated with 5000U/ml IFN alpha for 48 hours. Flow Cyt: Daudi cells treated with 5000U/ml IFN alpha for 48 hours. IP: Daudi treated with 5000U/ml IFN alpha for 48 hours whole cell lysate.

  • General notes

    Ab222492 is the carrier-free version of ab207414. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab222492 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    Immunocytochemistry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (Human Burkitt's lymphoma cell line) cells labeling MX1 with ab207414 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing negative staining on Daudi cell line. Expression was induced and showed cytoplasmic staining after cells were treated with IFN alpha (5000 U/ml) for 48h. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207414).

  • Flow Cytometry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    Flow Cytometry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Daudi (Human Burkitt's lymphoma cell line) treated with/without IFN-alpha (5000U/ml) for 48h cells labeling MX1 with ab207414 at 1/600 dilution (IFN alpha treated - red; untreated - green) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207414).

  • Immunoprecipitation - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    Immunoprecipitation - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)

    MX1 was immunoprecipitated from 0.35 mg of Daudi (Human Burkitt's lymphoma cell line) treated with IFN alpha (5000U/ml) for 48h whole cell lysate with ab207414 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab207414 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Daudi treated with IFN alpha (5000U/ml) for 48h whole cell lysate 10µg (Input).

    Lane 2: ab207414 IP in Daudi treated with IFN alpha (5000U/ml) for 48h whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207414 in Daudi treated with IFN alpha (5000U/ml) for 48h whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207414).

  • Immunocytochemistry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    Immunocytochemistry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)

    Clone EPR19967 (ab222492) has been successfully conjugated by Abcam. This image was generated using Anti-MX1 antibody [EPR19967] (Alexa Fluor® 488). Please refer to ab237298 for protocol details.

    ab237298 staining MX1 in Daudi cells +/- IFN alpha (5000 U/ml, 48h). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237298 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    Flow Cytometry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    This data was developed using ab207414, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20U/mL IFN alpha 1 for 24h cells labeling MX1 with purified ab207414 at 1/500 dilution ( 1µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    Immunocytochemistry - Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)

    Clone EPR19967 (ab222492) has been successfully conjugated by Abcam. This image was generated using Anti-MX1 antibody [EPR19967] (Alexa Fluor® 647). Please refer to ab237299 for protocol details.

    ab237299 staining MX1 in Daudi cells +/- IFN alpha (5000 U/ml, 48h). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237299 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)
    Anti-MX1 antibody [EPR19967] - BSA and Azide free (ab222492)

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