Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19967] to MX1
Suitable for: IP, Flow Cyt, ICC/IF, WB
Reacts with: Human

Product name

Anti-MX1 antibody [EPR19967]
See all MX1 primary antibodies
Description

Rabbit monoclonal [EPR19967] to MX1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi treated with 20U/ml IFN alpha 1 for 24 hours whole cell lysate; Human spleen and tonsil lysates; Untreated Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate ICC/IF: Daudi cells treated with 20U/ml IFN alpha 1 for 24 hours. IP: Daudi treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate Flow Cyt: Daudi cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19967
Isotype

IgG
Research areas

Western blot - Anti-MX1 antibody [EPR19967] (ab207414)

All lanes : Anti-MX1 antibody [EPR19967] (ab207414) at 1/2000 dilution

Lane 1 : Untreated Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 76 kDa
Observed band size: 76 kDa

Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST

Exposure time: 3.25 seconds
Immunocytochemistry/ Immunofluorescence - Anti-MX1 antibody [EPR19967] (ab207414)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (Human Burkitt's lymphoma cell line) cells labeling MX1 with ab207414 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing negative staining on Daudi cell line. Expression was induced and showed cytoplasmic staining after cells were treated with IFN alpha 1 (20 U/ml) for 24h. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-MX1 antibody [EPR19967] (ab207414)

Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST

All lanes : Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution

Lanes 1-2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207414 in Daudi treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP detection reagent (HRP))

Observed band size: 76 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-MX1 antibody [EPR19967] (ab207414)

Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20U/mL IFN alpha 1 for 24h cells labeling MX1 with purified ab207414 at 1/500 dilution ( 1µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). Untreated control (Green).
Western blot - Anti-MX1 antibody [EPR19967] (ab207414)

All lanes : Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution

Lane 1 : Human spleen lysate
Lane 2 : Human tonsil lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 76 kDa
Observed band size: 76 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 seconds; Lane 2: 1 minute.
Anti-MX1 antibody [EPR19967] (ab207414)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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