Anti-Mucin 5AC antibody [1-13M1] (ab24070)
![Anti-Mucin 5AC antibody [1-13M1] (ab24070)](https://www.abcam.com/ps/products/24/ab24070/Images/ab24070-34373-anti-mucin-5ac-antibody-1-13m1-flow-cytometry.jpg "Anti-Mucin 5AC antibody [1-13M1] (ab24070)")
Key features and details
- Mouse monoclonal [1-13M1] to Mucin 5AC
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Mucin 5AC antibody [1-13M1]
See all Mucin 5AC primary antibodies -
Description
Mouse monoclonal [1-13M1] to Mucin 5AC -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification) (Human).
-
Epitope
The epitope is destroyed by beta-mercaptoethanol. -
Positive control
- Human intestine and stomach
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1-13M1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
-
Flow Cytometry - Anti-Mucin 5AC antibody [1-13M1] (ab24070)Overlay histogram showing A549 cells stained with ab24070 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24070, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
