Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-mTOR antibody [EPR390(N)] - BSA and Azide free (ab248718)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR390(N)] to mTOR - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-mTOR antibody [EPR390(N)] - BSA and Azide free
    See all mTOR primary antibodies

  • Description

    Rabbit monoclonal [EPR390(N)] to mTOR - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 cell lysate.

  • General notes

    ab248718 is the carrier-free version of ab134903. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248718 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-mTOR antibody [EPR390(N)] - BSA and Azide free (ab248718)
    Western blot - Anti-mTOR antibody [EPR390(N)] - BSA and Azide free (ab248718)
    All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/10000 dilution

    Lane 1 : Wild-type HEK-293 cell lysate
    Lane 2 : MTOR knockout HEK-293 cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : MTOR knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 289 kDa
    Observed band size: 289 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab134903).

    Lanes 1 - 4: Merged signal (red and green). Green - ab134903 observed at 289 kDa. Red - loading control, ab130007 observed at 125 kDa.

     ab134903 was shown to react with mTOR in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255411 (knockout cell lysate ab263789) was used. Wild-type and mTOR knockout samples were subjected to SDS-PAGE. ab134903 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Anti-mTOR antibody [EPR390(N)] - BSA and Azide free (ab248718)
    Anti-mTOR antibody [EPR390(N)] - BSA and Azide free (ab248718)

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