Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI2F10] to MSI2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-MSI2 antibody [OTI2F10]
See all MSI2 primary antibodies
Description

Mouse monoclonal [OTI2F10] to MSI2
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human MSI2. Produced in HEK-293T cells. NP_620412
Database link: Q96DH6
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY MSI2 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC12 and MCF7 cell lysates. IHC-P: Human ovary adenocarcinoma, pancreas, pancreas carcinoma, endometrium adenocarcinoma and prostate tissues. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY MSI2; HeLa cells.
General notes

The clone number has been updated from 2F10 to OTI2F10, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS, 1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI2F10
Isotype

IgG1
Research areas

Western blot - Anti-MSI2 antibody [OTI2F10] (ab156770)

All lanes : Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/200 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : SVT2 (Mouse cell line) cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : MDCK (Canine kidney cell line) cell lysate
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell lysate

Lysates/proteins at 35 µg per lane.

Predicted band size: 35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [OTI2F10] (ab156770)

Immunofluorescent analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY MSI2, labeling MSI2 with ab156770 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (ab156770)

Paraffin-embedded human ovary adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [OTI2F10] (ab156770)

Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MSI2 with ab156770 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (ab156770)

Paraffin-embedded human pancreas tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (ab156770)

Paraffin-embedded human pancreas adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (ab156770)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (ab156770)

Paraffin-embedded human prostate tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Western blot - Anti-MSI2 antibody [OTI2F10] (ab156770)

All lanes : Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/4000 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY MSI2 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 35 kDa

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