Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)
![Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)](https://www.abcam.com/ps/products/250/ab250439/Images/ab250439-405871-anti-mrrf-antibody-epr14140-b-bsa-and-azide-free-western-blot.jpg "Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14140(B)] to MRRF - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free
See all MRRF primary antibodies -
Description
Rabbit monoclonal [EPR14140(B)] to MRRF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250439 is the carrier-free version of ab181223. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250439 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14140(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)All lanes : Anti-MRRF antibody [EPR14140(B)] (ab181223) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 29 kDaThis data was developed using ab181223, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)](https://www.abcam.com/ps/products/250/ab250439/Images/ab250439-2-anti-mrrf-antibody-epr14140-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)This data was developed using ab181223, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cardiac muscle tissue sections labeling MRRF using ab181223 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)](https://www.abcam.com/ps/products/250/ab250439/Images/ab250439-1-anti-mrrf-antibody-epr14140-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)This data was developed using ab181223, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling MRRF using ab181223 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)](https://www.abcam.com/ps/products/250/ab250439/Images/ab250439-4-benefits-of-recombinant-antibodies.png)
Anti-MRRF antibody [EPR14140(B)] - BSA and Azide free (ab250439)
