Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)
![Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)](https://www.abcam.com/ps/products/250/ab250927/Images/ab250927-406381-anti-mrp2-antibody-epr10997-2-bsa-and-azide-free-western-blot-human.jpg "Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10997(2)] to MRP2 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free
See all MRP2 primary antibodies -
Description
Rabbit monoclonal [EPR10997(2)] to MRP2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250927 is the carrier-free version of ab187644. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250927 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10997(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)All lanes : Anti-MRP2 antibody [EPR10997(2)] (ab187644) at 1/1000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC2 knockout A549 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 174 kDaThis data was developed using ab187644, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab187644 observed at 210 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab187644 was shown to recognize MRP2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab187644 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)](https://www.abcam.com/ps/products/250/ab250927/Images/ab250927-2-anti-mrp2-antibody-epr10997-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)This data was developed using ab187644, the same antibody clone in a different buffer formulation.Immunohistochemistry analysis of paraffin-embedded Human liver tissue labeling MRP2 with ab187644 at 1/1000 dilution. A prediluted HRP polymer for Rabbit IgG used as secondary antibody. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)](https://www.abcam.com/ps/products/250/ab250927/Images/ab250927-398961-anti-mrp2-antibody-epr10997-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)All lanes : Anti-MRP2 antibody [EPR10997(2)] (ab187644) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : A549 lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 174 kDaThis data was developed using ab187644, the same antibody clone in a different buffer formulation.
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![Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)](https://www.abcam.com/ps/products/250/ab250927/Images/ab250927-4-benefits-of-recombinant-antibodies.png)
Anti-MRP2 antibody [EPR10997(2)] - BSA and Azide free (ab250927)
