Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2429(2)] to Moesin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free
    See all Moesin primary antibodies

  • Description

    Rabbit monoclonal [EPR2429(2)] to Moesin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa cell lysate. ICC: HeLa cells. WB: HeLa cell lysate.

  • General notes

    ab249530 is the carrier-free version of ab169789.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    Western blot - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    All lanes : Anti-Moesin antibody [EPR2429(2)] (ab169789) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    This data was developed using ab169789, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    Immunoprecipitation - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    This data was developed using ab169789, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of pellet from HeLa cell lysate, using anti-Moesin ab169789 at a 1/10 dilution.
  • Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    Flow Cytometry - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    This data was developed using ab169789, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Raji cells labeling ab169789 (red) at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    This data was developed using ab169789, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung tissue, labeling Moesin using ab169789 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    This data was developed using ab169789, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human stomach tissue, labeling Moesin using ab169789 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)
    Anti-Moesin antibody [EPR2429(2)] - BSA and Azide free (ab249530)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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