Anti-MKK7 (phospho S271 + T275) antibody (ab4762)
Key features and details
- Rabbit polyclonal to MKK7 (phospho S271 + T275)
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-MKK7 (phospho S271 + T275) antibody
See all MKK7 primary antibodies -
Description
Rabbit polyclonal to MKK7 (phospho S271 + T275) -
Host species
Rabbit -
Specificity
This antibody does not react with other MEK isoforms. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human MKK7.
(Peptide available asab5742) -
Positive control
- Fusion protein expressing MEK 7.
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General notes
This product was previously labelled as MEK7
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 7. The final product is generated by affinity chromatography using an MEK 7 derived peptide that is phosphorylated at serine 271 and threonine 275. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)
Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50
µ
