Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MKK7 antibody [EP1455Y] (ab52618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1455Y] to MKK7
  • Suitable for: WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MKK7 antibody [EP1455Y]
    See all MKK7 primary antibodies

  • Description

    Rabbit monoclonal [EP1455Y] to MKK7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MKK7 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysate, human urinary bladder tissue Flow Cyt: MCF7 cells.

  • General notes

    This product was previously labelled as MEK7.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)
    Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: MEK7 knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
    Lanes 4, 8 and 12: A431 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target - ab52618 observed at 47 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal
    ab52618 was shown to specifically react with MEK7 when MEK7 knockout samples were used. Wild-type and MEK7 knockout samples were subjected to SDS-PAGE. ab52618 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-MKK7 antibody [EP1455Y] (ab52618)
    Flow Cytometry - Anti-MKK7 antibody [EP1455Y] (ab52618)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MKK7 with ab52618 at 1/100 dilution (1 µg) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)
    Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)
    Anti-MKK7 antibody [EP1455Y] (ab52618) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] (ab52618)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] (ab52618)

    Ab52618 at 1/50 dilution staining human baldder tissue; paraffin embedded.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-MKK7 antibody [EP1455Y] (ab52618)
    Anti-MKK7 antibody [EP1455Y] (ab52618)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MKK7 antibody [EP1455Y] (ab52618)

© 2021 Astana Biomed Group LLP. All rights reserved.