Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070)
![Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070)](https://www.abcam.com/ps/products/276/ab276070/Images/ab276070-2-AntiMID1IP1-antibody-005-icc-HeLa-cells.jpg "Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [005] to MID1IP1 - BSA and Azide free
- Suitable for: WB, ICC
- Reacts with: Human
Overview
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Product name
Anti-MID1IP1 antibody [005] - BSA and Azide free
See all MID1IP1 primary antibodies -
Description
Rabbit monoclonal [005] to MID1IP1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human MID1IP1 aa 1-183.
Database link: Q9NPA3 -
Positive control
- WB: HepG2 whole cell lysate. ICC: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
005 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-MID1IP1 antibody [005] (ab276070)
Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling MID1IP1 with ab276070 at 1:60 dilution, 4°C overnight, followed by Goat Anti-rabbit IgG secondary antibody Alexa Fluor©488-conjugated (green). Positive staining was localized to Cytoskeleton. Cells were blocked with 10% serum prior incubation.
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![Western blot - Anti-MID1IP1 antibody [005] (ab276070)](https://www.abcam.com/ps/products/276/ab276070/Images/ab276070-1-anti-mid1ip1-antibody-wb-hepg2-cells.jpg)
Western blot - Anti-MID1IP1 antibody [005] (ab276070)Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 20 kDa
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![Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070)](https://www.abcam.com/ps/products/276/ab276070/Images/ab276070-3-benefits-of-recombinant-antibodies.png)
Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070)
