Anti-GRWD1 antibody - BSA and Azide free (ab188419)
Key features and details
- Rabbit polyclonal to GRWD1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRWD1 antibody - BSA and Azide free
See all GRWD1 primary antibodies -
Description
Rabbit polyclonal to GRWD1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant full length protein (proprietary-tag) corresponding to Human GRWD1 aa 1 to the C-terminus. (Purified from E. coli).
Database link: Q9BQ67 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine) -
Carrier free
Yes -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab188419 is filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT116 cells labeling GRWD1 with ab188419 at 1/500 dilution (green), followed by goat anti-rabbit IgG conjugated with Alex488 at 1/2000 dilution (green). DAPI was used to stain the cell nuclei (blue) and anti-fibrillarin (red) antibodies as a marker for nucleoli. Right panel shows merged images.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling GRWD1 with ab188419 at 1/1000 dilution, followed by goat anti-rabbit IgG conjugated with Alex488 at 1/500 dilution (red). DAPI was used to stain the cell nuclei (blue). Right panel shows merged images.
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All lanes : Anti-GRWD1 antibody - BSA and Azide free (ab188419) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate untreated
Lane 2 : HeLa whole cell lysate treated with adriamycn
Lane 3 : NIH 3T3 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?