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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules

Anti-KIF14 antibody (ab71155)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-KIF14 antibody (ab71155)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to KIF14
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KIF14 antibody
    See all KIF14 primary antibodies
  • Description

    Rabbit polyclonal to KIF14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1600 and the C-terminus (residue 1648) of human KIF14 (NP_055690.1).

  • Positive control

    • Whole cell lysates from Hela cells and 293T cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin-like

Images

  • Western blot - Anti-KIF14 antibody (ab71155)
    Western blot - Anti-KIF14 antibody (ab71155)
    All lanes : Anti-KIF14 antibody (ab71155) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Predicted band size: 186 kDa
    Observed band size: 210 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 180 kDa, 190 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF14 antibody (ab71155)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF14 antibody (ab71155)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling KIF14 with ab71155 at 1/200 (1µg/ml). Detection: DAB.
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF14 antibody (ab71155)
    Immunocytochemistry/ Immunofluorescence - Anti-KIF14 antibody (ab71155)
    Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling KIF14 with ab71155 at 1/100 (2µg/ml). A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • Immunoprecipitation - Anti-KIF14 antibody (ab71155)
    Immunoprecipitation - Anti-KIF14 antibody (ab71155)
    Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab71155 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab71155 at 1 µg/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: IHC-P, IP, WB

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