Anti-MAP1B antibody (ab224115)
Key features and details
- Rabbit polyclonal to MAP1B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP1B antibody
See all MAP1B primary antibodies -
Description
Rabbit polyclonal to MAP1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MAP1B aa 1300-1500.
Database link: P46821 -
Positive control
- IHC-P: Human testis, cerebral cortex and cerebellum tissue; ICC: U-2 OS whole cells; WB: siRNA transfected U-251MG whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAP1B antibody (ab224115)All lanes : Anti-MAP1B antibody (ab224115) at 0.4 µg/ml
Lane 1 : Control siRNA transfected - U-251 MG (human brain glioma cell line) whole cell lysate
Lane 2 : siRNA probe#1 transfected - U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : siRNA probe#2 transfected - U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 271 kDaLoading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)Immunohistochemical analysis of human testis tissue labeling MAP1B in the membrane of cells in seminiferous ducts at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody (ab224115)Immunocytochemical analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling MAP1B in the cytosol at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)Immunohistochemical analysis of human testis tissue labeling MAP1B in the membrane of cells in granular layer at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)Immunohistochemical analysis of human cerebral cortex tissue labeling MAP1B in the cytoplasm of neurones at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1B antibody (ab224115)Immunohistochemical analysis of human skeletal muscle tissue labeling MAP1B in mytocytes (low positivity) at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
