Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MICA antibody [3H5] - BSA and Azide free (ab278536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [3H5] to MICA - BSA and Azide free
  • Suitable for: Flow Cyt, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-MICA antibody [3H5] - BSA and Azide free
    See all MICA primary antibodies

  • Description

    Mouse monoclonal [3H5] to MICA - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Flow Cyt: HeLa cells. ELISA: Human MICA (aa 24-307).

  • General notes

    ab278536 is the carrier-free version of ab278534. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278536 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-MICA antibody [3H5] - BSA and Azide free (ab278536)
    Flow Cytometry - Anti-MICA antibody [3H5] - BSA and Azide free (ab278536)

    This data was developed using ab278534 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / HeLa (Human cervix adenocarcinoma epithelial cell, Right) cells labelling MICA with ab278534 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Jurkat.

  • ELISA - Anti-MICA antibody [3H5] - BSA and Azide free (ab278536)
    ELISA - Anti-MICA antibody [3H5] - BSA and Azide free (ab278536)

    This data was developed using ab278534 the same antibody clone in a different buffer formulation.

    Human MICA（aa 24-307) and Human MICB（aa 24-297）= 1000 ng/ml

    ab278534 used at 0 - 4000 ng/ml.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）secondary antibody was used at 1:1000 dilution. 

  • Anti-MICA antibody [3H5] - BSA and Azide free (ab278536)
    Anti-MICA antibody [3H5] - BSA and Azide free (ab278536)

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