Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2762(2)] to Mib1/Mindbomb
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Mib1/Mindbomb antibody [EPR2762(2)]
    See all Mib1/Mindbomb primary antibodies

  • Description

    Rabbit monoclonal [EPR2762(2)] to Mib1/Mindbomb

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Mib1/Mindbomb aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • K562, HeLa, Caco2, NCCIT, and Human brain lysates; Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution

    Lane 1 : Human fetal brain tissue
    Lane 2 : K562 (human chronic myelogenous leukemia) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 110 kDa
    Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.



    Blocking and diluting buffer: 5% NFDM/TBST

    This image was produced using purified antibody. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) This image is courtesy of an Abreview submitted by Mr Rudolf Jung

    Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.

    Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

    ab124929 staining Mib1/Mindbomb in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab124929 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This image was produced using purified antibody. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

    ab124929 staining Mib1/Mindbomb in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This image was produced using purified antibody. 

  • Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilution + Mouse spleen tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 110 kDa
    Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.



    Diluting and blocking buffer: 5% NFDM /TBST.

    This image was produced using purified antibody. 

  • Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution + Mouse brain tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 110 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    This image was produced using purified antibody. 

  • Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : Caco2 cell lysates
    Lane 4 : NCCIT cell lysates
    Lane 5 : Human brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 110 kDa



    This image was produced using unpurified antibody. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

    ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.

    This image was produced using unpurified antibody. 

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This image was produced using unpurified antibody. 

  • Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)
    Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

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