Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (ab250688)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13540] to mGluR1a - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-mGluR1a antibody [EPR13540] - BSA and Azide free
    See all mGluR1a primary antibodies

  • Description

    Rabbit monoclonal [EPR13540] to mGluR1a - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250688 is the carrier-free version of ab183712 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250688 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (ab250688)
    Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (ab250688)
    All lanes : Anti-mGluR1a antibody [EPR13540] (ab183712) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate boiled
    Lane 2 : Mouse brain lysate, boiled
    Lane 3 : Rat brain tissue lysate, boiled
    Lane 4 : Human cerebellum lysate unboiled
    Lane 5 : Mouse brain lysate, unboiled
    Lane 6 : Rat brain tissue lysate, unboiled

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 132 kDa
    Observed band size: 101,132 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    This data was developed using ab183712, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in PMID: 30375476 and 11672421.

    We recommend not to boil the samples after lysis to get desired WB results.

  • Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (ab250688)
    Western blot - Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (ab250688)
    Anti-mGluR1a antibody [EPR13540] (ab183712) at 1/5000 dilution + Human cerebellum lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 132 kDa
    Observed band size: 101,200 kDa why is the actual band size different from the predicted?



    This data was developed using ab183712, the same antibody clone in a different buffer formulation.

     

  • Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (ab250688)
    Anti-mGluR1a antibody [EPR13540] - BSA and Azide free (ab250688)

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