Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal to MEK2
Suitable for: WB, IP, IHC-P, ICC
Reacts with: Mouse, Human

Product name

Anti-MEK2 antibody
See all MEK2 primary antibodies
Description

Rabbit monoclonal to MEK2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human MEK2 aa 1-50. The exact sequence is proprietary. NP_109587.1
Database link: P36507
Positive control
- ICC: KG-1 cells. IHC-P: Human prostate carcinoma and breast carcinoma tissue. WB: HepG2, HEK-293T, A549, Jurkat, RKO, U-2 OS, LNCaP, HeLa, NIH/3T3 and TCMK whole cell lysate.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Isotype

IgG
Research areas

Western blot - Anti-MEK2 antibody (ab265586)

All lanes : Anti-MEK2 antibody (ab265586) at 1/1000 dilution

Lane 1 : HepG2 whole cell lysate
Lane 2 : HEK-293T whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : RKO whole cell lysate
Lane 6 : U-2 OS whole cell lysate
Lane 7 : LNCaP whole cell lysate
Lane 8 : HeLa whole cell lysate
Lane 9 : NIH/3T3 whole cell lysate
Lane 10 : TCMK whole cell lysate

Lysates/proteins at 50 µg per lane.

Predicted band size: 44 kDa

Exposure time: 3 minutes

Cells prepared using NETN lysis buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody (ab265586)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling MEK2 with ab265586 at 1/100 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody (ab265586)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling MEK2 with ab265586 at 1/100 dilution. Detection: DAB.
Immunocytochemistry - Anti-MEK2 antibody (ab265586)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded KG-1 cells labeling MEK2 with ab265586 at 1/100 dilution. Detection: DAB.
Immunoprecipitation - Anti-MEK2 antibody (ab265586)

MEK2 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265586 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265586 at 1/1000 dilution.
Lane 1: ab265586 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.

Exposure time: 30 secs.

Cells prepared using NETN lysis buffer.
Anti-MEK2 antibody (ab265586)

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