Anti-MEK2 antibody (ab265601)
Key features and details
- Rabbit polyclonal to MEK2
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEK2 antibody
See all MEK2 primary antibodies -
Description
Rabbit polyclonal to MEK2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog
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Immunogen
Synthetic peptide within Human MEK2 aa 1-50. The exact sequence is proprietary. NP_109587.1
Database link: P36507 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody (ab265601)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling MEK2 with ab265601 at 1/200 dilution. Detection: DAB.
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Western blot - Anti-MEK2 antibody (ab265601)All lanes : Anti-MEK2 antibody (ab265601) at 0.04 mg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-MEK2 antibody (ab265601)MEK2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab265601 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitates using ab265601 at 1 µg/ml.
Lane 1: ab265601 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
