Anti-MASPIN antibody (ab272858)
Key features and details
- Rabbit polyclonal to MASPIN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MASPIN antibody
See all MASPIN primary antibodies -
Description
Rabbit polyclonal to MASPIN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human MASPIN. The exact sequence is proprietary. Center region.
Database link: P36952 -
Positive control
- WB: NIH/3T3 and HeLa S3 whole cell lysate. IHC-P: H1299 xenograft.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MASPIN antibody (ab272858)All lanes : Anti-MASPIN antibody (ab272858) at 1/500 dilution
Lane 1 : WT HeLa cell extracts
Lane 2 : MASPIN KO HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG10% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (ab272858)Paraffin embedded H1299 xenograft tissue stained for MASPIN using ab272858 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-MASPIN antibody (ab272858)Anti-MASPIN antibody (ab272858) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg10% SDS-PAGE
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Western blot - Anti-MASPIN antibody (ab272858)Anti-MASPIN antibody (ab272858) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg10% SDS-PAGE