Anti-Heme Oxygenase 1 antibody (ab85309)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Heme Oxygenase 1 antibody
See all Heme Oxygenase 1 primary antibodies -
Description
Rabbit polyclonal to Heme Oxygenase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat Heme Oxygenase 1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94374) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab85309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). IHC-P Use at an assay dependent concentration. Target
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Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
Sequence similarities
Belongs to the heme oxygenase family. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Entrez Gene: 24451 Rat
- Omim: 141250 Human
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
- SwissProt: P06762 Rat
- Unigene: 517581 Human
see all -
Alternative names
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
see all
Images
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Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : HEK-293
Lane 2 : HL60
Lane 3 : Rt spleen
Lane 4 : NR8383
Lane 5 : Ms spleen
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti Rabbit
Predicted band size: 33 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab85309)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C) on human lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins at 21°C. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: Positivity appears to be confined to macrophages- the primary antibody was omitted on the sequential section and was negative for macrophages ( to rule out lipofuscin).
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Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Spleen (Rat) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 47 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 :Mouse lung normal tissue lysate - total protein (ab29297)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab85309)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C)on rat lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: As well as in macrophage cytoplasm, there is also clear positivity in the lining epitheium of the respiratory conducting tubes. I do not know if this is specific/non-specific.
Protocols
References (3)
ab85309 has been referenced in 3 publications.
- Li YY et al. Protective effects of HO-1 pathway on lung injury subsequent to limb ischemia reperfusion. Kaohsiung J Med Sci 35:417-424 (2019). PubMed: 30977589
- Chen X et al. Ethanol extract of Schisandrae chinensis fructus ameliorates the extent of experimentally induced atherosclerosis in rats by increasing antioxidant capacity and improving endothelial dysfunction. Pharm Biol 56:612-619 (2018). PubMed: 31070526
- Kataoka K et al. Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice. Int J Mol Sci 17:N/A (2016). IHC-P ; Mouse . PubMed: 27854327
Images
-
Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : HEK-293
Lane 2 : HL60
Lane 3 : Rt spleen
Lane 4 : NR8383
Lane 5 : Ms spleen
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti Rabbit
Predicted band size: 33 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab85309) This image is courtesy of Carl Hobbs, King's College London, United Kingdom
Immunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C) on human lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins at 21°C. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: Positivity appears to be confined to macrophages- the primary antibody was omitted on the sequential section and was negative for macrophages ( to rule out lipofuscin).
-
Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Spleen (Rat) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 47 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
-
Western blot - Anti-Heme Oxygenase 1 antibody (ab85309)All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 :Mouse lung normal tissue lysate - total protein (ab29297)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab85309) This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C)on rat lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: As well as in macrophage cytoplasm, there is also clear positivity in the lining epitheium of the respiratory conducting tubes. I do not know if this is specific/non-specific.
