Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [MEK12S221-D3] to MEK1 (phospho S221) + MEK2 (phospho S221)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3]
Description

Rabbit monoclonal [MEK12S221-D3] to MEK1 (phospho S221) + MEK2 (phospho S221)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human MEK1 (phospho S221). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Uniprot Q02750/ P36507
Database link: Q02750

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Positive control
- WB: NIH/3T3, C2C12, HT1080 and A549 (each treated with TPA) cell extracts. Flow cyt: HeLa cells (treated with pervanadate).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

MEK12S221-D3
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278564 at 0.1 µg/mL.
Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

All lanes : Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564) at 0.1 µg/ml

Lane 1 : A549 cell extract treated with TPA
Lane 2 : A549 cell extract untreated with TPA
Flow Cytometry - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate (green) or imatinib and blocked with phospho peptide (gold) or pervanadate and blocked with phospho peptide (black) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278564 at 0.1 µg/mL.
Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

All lanes : Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564) at 0.1 µg/ml

Lane 1 : HT1080 cell extract treated with TPA
Lane 2 : HT1080 cell extract untreated with TPA
Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

Western blot analysis of C2C12 cell extract untreated or treated with TPA using 0.1 µg/mL ab278564.
Western blot - Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

All lanes : Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564) at 0.1 µg/ml

Lane 1 : NIH3T3 cell extract untreated with TPA
Lane 2 : NIH3T3 cell extract treated with TPA
Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEK1 (phospho S221) + MEK2 (phospho S221) antibody [MEK12S221-D3] (ab278564)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Light chain type

Research areas

Images