Anti-ALDH1A1 antibody (ab227948)
Key features and details
- Rabbit polyclonal to ALDH1A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH1A1 antibody
See all ALDH1A1 primary antibodies -
Description
Rabbit polyclonal to ALDH1A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Cow, Dog, Chimpanzee -
Immunogen
Recombinant fragment within Human ALDH1A1 (internal sequence). The exact sequence is proprietary.
Database link: P00352 -
Positive control
- WB: A549 whole cell lysate; Mouse and rat liver lysates. IHC-P: Cal27 xenograft tissue. ICC/IF: A549 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded Cal27 xenograft tissue stained for ALDH1A1 using ab227948 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based, pH 8.0 buffer, 15 minutes.
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Anti-ALDH1A1 antibody (ab227948) at 1/5000 dilution + Rat liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa10% SDS-PAGE gel.
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Methanol-fixed A549 (human lung carcinoma cell line) cells stained for ALDH1A1 (green) using ab227948 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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Anti-ALDH1A1 antibody (ab227948) at 1/10000 dilution + Mouse liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa10% SDS-PAGE gel.
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Anti-ALDH1A1 antibody (ab227948) at 1/5000 dilution + A549 (human lung carcinoma cell line) whole cell lysate) at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa10% SDS PAGE gel.