Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1933Y] to ALDH1A1 - BSA and Azide free
  • Suitable for: ICC, WB, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free
    See all ALDH1A1 primary antibodies

  • Description

    Rabbit monoclonal [EP1933Y] to ALDH1A1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HepG2 cell lysate; IHC-P: Human liver tissue, Human bladder carcinomaFlow Cyt: HepG2 cells

  • General notes

    ab215996 is the carrier-free version of ab52492 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab215996 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling ALDH1A1 with purified ab52492 at 1/50 dilution (3.54 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    ab52492 (purified) at 1/20 dilution (2ug) immunoprecipitating ALDH1A1 in HepG2 whole cell lysates.
    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab52492 & HepG2 whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52492 in HepG2 whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Immunocytochemistry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunocytochemistry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ALDH1A1 with ab52492 (purified) at 1/500 dilution (4 μg/ml).

    Cells were fixed in 100% methanol. ab150077, an AlexaFluor®488 Goat anti-Rabbit secondary antibody was used at 1/1000 dilution (2 μg/ml). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 1/200 dilution (2.5 μg/ml). DAPI was used as nuclear counterstain.

    Confocal image showing cytoplasmic staining on HepG2 cell line.

    Negative control: No staining on MCF-7 cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Flow Cytometry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Flow Cytometry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Clone EP1933Y (ab215996) has been successfully conjugated by Abcam. This image was generated using Anti-ALDH1A1 antibody [EP1933Y] (PE). Please refer to ab209437 for protocol details.

    Overlay histogram showing MCF7 cells stained with ab209437 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min at 22°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209437, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Immunocytochemistry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunocytochemistry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Clone EP1933Y (ab215996) has been successfully conjugated by Abcam. This image was generated using Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647). Please refer to ab195255 for protocol details.

    ab195255 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195255 at a working dilution of 1 in 50 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunocytochemistry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Clone EP1933Y (ab215996) has been successfully conjugated by Abcam. This image was generated using Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 488). Please refer to ab195254 for protocol details.

    ab195254 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195254 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Flow Cytometry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ALDH1A1 with purified ab52492 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996) Image from Gong et al PLoS One. 2010 Dec 20;5(12):e15630. doi: 10.1371/journal.pone.0015630. Fig 1.

    Tumor tissues of primary invasive ductal carcinomas of the breast were obtained from 192 female patients with stage IIB and III prior to pre-operative neoadjuvant chemotherapy.

    (progressive or stable disease, PD/SD)

    (partial or complete remission, PR/CR)

    The level of ALDH1 was tested by immunohistochemistry staining in paraffin-embedded tissue sections. Rabbit monoclonal ALDH1A1 antibody (ab52492, unpurified, Abcam) used at a 1:100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Flow Cytometry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Flow Cytometry - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab52492 (unpurified) (red line).

    The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Immunohistochemical analysis of paraffin-embedded human liver tissue sections labeling ALDH1A1 with ab52492 (unpurified) at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human brain tissue sections labeling ALDH1A1 with ab52492 (unpurified).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human lung tissue sections labeling ALDH1A1 with ab52492 (unpurified).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human kidney tissue sections labeling ALDH1A1 with ab52492 (unpurified).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52492).

  • Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
    Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)

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