Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-mCherry antibody [EPR20579] (ab213511)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20579] to mCherry
  • Suitable for: ELISA, IP, WB
  • Reacts with: Species independent

Overview

  • Product name

    Anti-mCherry antibody [EPR20579]
    See all mCherry primary antibodies

  • Description

    Rabbit monoclonal [EPR20579] to mCherry

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T cells transfected with a His-tagged mCherry whole cell lysate. IP: 293T cells transfected with a His-tagged mCherry whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20579

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    All lanes : Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution

    Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty expression vector whole cell lysate
    Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution + Green fluorescent protein (GFP) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Green fluorescent protein (GFP) contains aa1-238 with His-tag.

  • Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)
    Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)

    mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1:  293T transfected with a His-tagged mCherry whole cell lysate 10 µg (Input). 

    Lane 2: ab213511 IP in 293T transfected with a His-tagged mCherry whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213511 in 293T transfected with a His-tagged mCherry whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-mCherry antibody [EPR20579] (ab213511)
    Anti-mCherry antibody [EPR20579] (ab213511)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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