Anti-mCherry antibody [1C51] (ab125096)
![Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.com/ps/products/125/ab125096/Images/ab125096-318506-anti-mcherry-antibody-1c51-immunohistochemistry.jpg "Anti-mCherry antibody [1C51] (ab125096)")
Key features and details
- Mouse monoclonal [1C51] to mCherry
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG2a
Overview
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Product name
Anti-mCherry antibody [1C51]
See all mCherry primary antibodies -
Description
Mouse monoclonal [1C51] to mCherry -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to mCherry.
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Positive control
- ICC/IF: pFin-EF1-mCherry transfected HEK-293T cells. Rat bladder. IHC-Fr: Human pancreatic islet tissue; Rat brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1C51 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096) Baumgartner R et al. The RNA-binding proteins FMR1, rasputin and caprin act together with the UBA protein lingerer to restrict tissue growth in Drosophila melanogaster. PLoS Genet 9:e1003598 (2013).
4% paraformaldehyde fixed (at RT for 20 min and blocked with 2% NDS in 0.3% PBT) Drosophila melanogaster eye imaginal discs stained for mCherry (Red) using ab125096.
Analysis of rin hypomorphic alleles and the genomic rescue transgene GrinCherry. (A–C'') Negatively marked 72 h old rin2 (A, A''), (C, C'') mutant clones (induced with the FLP/FRT system) in eye imaginal discs of third instar larvae. Rin-Cherry levels expressed from the GrinCherry are autonomously increased in the rin mutant clones (A' and C'). The scale bar represents 50 µm.
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![Western blot - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.com/ps/products/125/ab125096/Images/ab125096-318532-anti-mcherry-antibody-1c51-western-blot.jpg)
Western blot - Anti-mCherry antibody [1C51] (ab125096)All lanes : Anti-mCherry antibody [1C51] (ab125096) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : HEK-293 cells transfected with mCherry-HA construct
Lane 4 : mCherry recombinant protein
Lane 5 : GFP recombinant protein
Lane 6 : HEK-293 transfected with GFP construct.Major band at about 30kDa corresponds to mCherry protein.
MCA-1C51 antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals band at 60kDa seen only in cell lysates.
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![Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.com/ps/products/125/ab125096/Images/ab125096-139267-anti-mcherry-antibody-1c51-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [1C51] (ab125096)mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 followed with a green secondary. DAPI was used to stain nuclei in blue. Cells are visualized using a confocal microscope. Transfected cells appear in yellow/orange colour.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096)](https://www.abcam.com/ps/products/125/ab125096/Images/ab125096-262964-anti-mcherry-antibody-1c51-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (ab125096) Image courtesy of an anonymous Abreviewab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.
