Anti-mCherry antibody (ab183628)
Key features and details
- Rabbit polyclonal to mCherry
- Suitable for: ICC/IF, IHC - Wholemount, IP, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-mCherry antibody
See all mCherry primary antibodies -
Description
Rabbit polyclonal to mCherry -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC - Wholemount, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to mCherry.
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Positive control
- IHC-Wm: 2 days-post-fertilization zebrafish embryos . WB: Full length recombinant mCherry. mCherry-tagged protein expressing HEK-293T cell lysate. ICC/IF: mCherry fusion protein-transfected HeLa cells IP: HEK-293T
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628) Wang et al PLoS Pathog. 2018 Jul 20;14(7):e1007189. doi: 10.1371/journal.ppat.1007189. eCollection 2018 Jul. Fig 7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
RABV and mGluR2 are internalized into cells and transported together in early and late endosomes.
N2a (Mouse neuroblastoma cells) cells were stained by using the Tyramide Signal Amplification immunofluorescent method. N2a cells infected with ERA-N-mCherry for 20 minutes at 37°C were used to perform immunofluorescence staining for RABV antigen (red), mGluR2 (green), Rab5 or Rab7 (purple), and the cell nuclei (blue). Co-localization of the RABV-mGluR2 complex with Rab5 (E, F).
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IHC - Wholemount - Anti-mCherry antibody (ab183628)Whole mount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryos labeling mCherry-expressing cells with ab183628 at 1/100 dilution.
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Western blot - Anti-mCherry antibody (ab183628)All lanes : Anti-mCherry antibody (ab183628) at 1/2000 dilution
Lane 1 : Full length recombinant mCherry at 0.005 µg
Lane 2 : Full length recombinant mCherry at 0.01 µg
Lane 3 : Full length recombinant mCherry at 0.02 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 27 kDa
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Western blot - Anti-mCherry antibody (ab183628)All lanes : Anti-mCherry antibody (ab183628) at 1/5000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract
Lane 2 : Whole cell lysate/extract of mCherry-tagged protein expressing HEK-293T cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
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Immunoprecipitation - Anti-mCherry antibody (ab183628)Immunoprecipitation analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract using ab183628 at a 1/3000 dilution.
Lane 1: 20 μg whole cell lysate/extract of Cherry protein-expressing HEK-293T cells.
Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.
Lane 3: Immunoprecipitation of mCherry protein with 2.5 μg of ab183628.7.5% SDS-PAGE. An anti-rabbit IgG (HRP) was used as a secondary reagent.
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Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628)Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with Triton X-100 for 1 minute, then 4% paraformaldehyde for 30 minutes at room temperature. mCherry (green) was labeled using ab183628 at a dilution of 1/500.
Red: mCherry fusion protein is expressed in the transfected cell.
Blue: Hoechst 33342 staining. -
Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628)Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with Triton X-100 for 1 minute, then 4% paraformaldehyde for 30 minutes at room temperature. mCherry (green) was labeled using ab183628 at a dilution of 1/500.
Red: mCherry fusion protein is expressed in the transfected cell.
Blue: Hoechst 33342 staining.
