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Epigenetics and Nuclear Signaling DNA methylation Methylated DNA Binding

Anti-MBD3 antibody (ab91458)

Anti-MBD3 antibody (ab91458)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to MBD3
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MBD3 antibody
    See all MBD3 primary antibodies
  • Description

    Rabbit polyclonal to MBD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, OrangutanDoes not react with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 265 - 291 (

    ACAEDDDEED EEEEEEEPDP DPEMEHV

    ) of Human MBD3 (NP_003917.1).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa or 293T cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Binding

Images

  • Western blot - Anti-MBD3 antibody (ab91458)
    Western blot - Anti-MBD3 antibody (ab91458)
    All lanes : Anti-MBD3 antibody (ab91458) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD3 antibody (ab91458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD3 antibody (ab91458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MBD3 with ab91458 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-MBD3 antibody (ab91458)
    Immunoprecipitation - Anti-MBD3 antibody (ab91458)
    SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91458.
    20% of the immunoprecipitate was loaded per lane, and probed with ab91458 at 1µg/ml (lane 1) or a control IgG (lane2).
    Detection: chemoluminescence with an exposure time of 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ChIP, Flow Cyt, ICC/IF, WB

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    Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)

    Applications: ChIP, Flow Cyt, ICC/IF, WB

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    Alexa Fluor® 488 Anti-MBD3 antibody [EPR9913] (ab197858)

    Applications: Flow Cyt, ICC/IF

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