Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9913] to MBD3 - BSA and Azide free
  • Suitable for: WB, ChIP, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MBD3 antibody [EPR9913] - BSA and Azide free
    See all MBD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9913] to MBD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ChIP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240144 is the carrier-free version of ab157464. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240144 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling MBD3 with purified ab157464 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

    Chromatin was prepared from NIH/3T3 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab157464 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • Flow Cytometry - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Flow Cytometry - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Flow cytometric analysis of permeabilized 293T cells labeling MBD3 with ab157464 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab157464 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Immunofluorescent analysis of HeLa cells labeling MBD3 with ab157464 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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