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Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9913] to MBD3 - ChIP Grade
  • Suitable for: WB, ICC/IF, Flow Cyt, ChIP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MBD3 antibody [EPR9913] - ChIP Grade
    See all MBD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9913] to MBD3 - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Mouse
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human MBD3.

  • Positive control

    • HeLa, 293T, fetal brain and Y79 lysates; HeLa cells; Permeabilized 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Western blot - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ProteinX knockout HAP1 cell lysate (20 µg)
    Lane 3: Wild-type HAP1 cell lysate (20 µg)
    Lane 4: ProteinX knockout HAP1 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab157464 observed at 33 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab157464 was shown to recognize MBD3 when MBD3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MBD3 knockout samples were subjected to SDS-PAGE. ab157464 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling MBD3 with purified ab157464 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • ChIP - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    ChIP - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab157464 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Western blot - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Western blot - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    All lanes : Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : Y79 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa

  • ChIP - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    ChIP - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Chromatin was prepared from NIH/3T3 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab157464 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Immunofluorescent analysis of HeLa cells labeling MBD3 with ab157464 at 1/100 dilution.
  • Flow Cytometry - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Flow Cytometry - Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Flow cytometric analysis of permeabilized 293T cells labeling MBD3 with ab157464 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
  • Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)
    Anti-MBD3 antibody [EPR9913] - ChIP Grade (ab157464)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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