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Epigenetics and Nuclear Signaling DNA methylation Methylated DNA Binding

Anti-MeCP2 antibody [4B4] (ab252840)

Anti-MeCP2 antibody [4B4] (ab252840)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4B4] to MeCP2
  • Suitable for: WB, IHC-P
  • Reacts with: Rat

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Overview

  • Product name

    Anti-MeCP2 antibody [4B4]
    See all MeCP2 primary antibodies
  • Description

    Mouse monoclonal [4B4] to MeCP2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat brain nuclear fraction lysate. IHC-P: Rat cerebrum tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    4B4
  • Isotype

    IgG2c
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Binding
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Images

  • Western blot - Anti-MeCP2 antibody [4B4] (ab252840)
    Western blot - Anti-MeCP2 antibody [4B4] (ab252840)
    All lanes : Anti-MeCP2 antibody [4B4] (ab252840) at 1/1000 dilution

    Lane 1 : Rat brain cytoplasmic fraction
    Lane 2 : Rat brain nuclear fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.   

    Exposure time: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] (ab252840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MeCP2 antibody [4B4] (ab252840)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MeCP2 with ab252840 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Anti-MeCP2 antibody [4B4] (ab252840)
    Anti-MeCP2 antibody [4B4] (ab252840)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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