Anti-MeCP2 antibody [4B4] (ab252840)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [4B4] to MeCP2
- Suitable for: WB, IHC-P
- Reacts with: Rat
Overview
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Product name
Anti-MeCP2 antibody [4B4]
See all MeCP2 primary antibodies -
Description
Mouse monoclonal [4B4] to MeCP2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Rat -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat brain nuclear fraction lysate. IHC-P: Rat cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4B4 -
Isotype
IgG2c -
Research areas
Images
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All lanes : Anti-MeCP2 antibody [4B4] (ab252840) at 1/1000 dilution
Lane 1 : Rat brain cytoplasmic fraction
Lane 2 : Rat brain nuclear fraction
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MeCP2 with ab252840 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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