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Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [54H19L38] to MARVELD2/Tricellulin
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Dog, Human

Overview

  • Product name

    Anti-MARVELD2/Tricellulin antibody [54H19L38]
    See all MARVELD2/Tricellulin primary antibodies

  • Description

    Rabbit monoclonal [54H19L38] to MARVELD2/Tricellulin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human MARVELD2/Tricellulin aa 350 to the C-terminus.
    Database link: Q8N4S9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: Caco 2 cells; WB: Caco 2, HeLa, PANC1, MCF-7 and MDCK cell lysates IP: HeLa cells

  • General notes

     This product was previously labelled as MARVELD2

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    54H19L38

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    All lanes : Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567) at 2 µg

    Lane 1 : Untransfected HeLa cells
    Lane 2 : HeLa cells transfected with scrambled SiRNA
    Lane 3 : HeLa cells transfected with MARVELD2/Tricellulin siRNA.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) secondary antibody at 1/40000 dilution

    Predicted band size: 64 kDa

  • Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    All lanes : Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : PANC1 cell lysate
    Lane 3 : MCF-7 cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2500 dilution

    Predicted band size: 64 kDa

  • Immunoprecipitation - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    Immunoprecipitation - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)

    Tricellulin was immunoprecipitated using 5 µg of ab203567 from lysate of HeLa (Lane 3). Normal Rabbit IgG was used as a Isotype control (Lane 2). 10 % input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using ab203567 and Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:2500 dilution. Chemiluminescent detection was performed using ECL Western Blotting Substrate.

  • Immunocytochemistry/ Immunofluorescence - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    Immunocytochemistry/ Immunofluorescence - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)

    Immunofluorescence analysis of Tricellulin was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab203567 at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    Immunocytochemistry/ Immunofluorescence - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)

    Immunofluorescent analysis of Caco 2 cells labeling MARVELD2/Tricellulin using ab203567 at 8 μg/mL. Alexa Fluor® 488 goat anti-rabbit at 1/1000 was used as secondary antibody (green). DAPI staining of nucleus (top left, blue), cell-cell junctions were labeled using a anti- Occludin antibody (top right, red). Bottom right: merged image.

  • Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    Western blot - Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    All lanes : Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567) at 3 µg/ml

    Lane 1 : Caco 2 cell lysate
    Lane 2 : MDCK cell lysate

    Predicted band size: 64 kDa

  • Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)
    Anti-MARVELD2/Tricellulin antibody [54H19L38] (ab203567)

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