Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1983Y] to MAP1LC3A - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free
    See all MAP1LC3A primary antibodies

  • Description

    Rabbit monoclonal [EP1983Y] to MAP1LC3A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239849 is the carrier-free version of ab52768.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)

    Immunocytochemistry/Immunofluorescence analysis of C6 (rat glioma) labelling MAP1LC3A with purified ab52768 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52768).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849) Image from Buckingham EM et al. Biotechniques. 2014;57(5):241-4. Fig 1.; doi: 10.2144/000114226.

    Immunocytochemistry/Immunofluorescence analysis of human keratinocytes (pannel A) and VZV-infected melanoma cells (panel E) labelling MAP1LC3A with ab52768.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52768).

  • Flow Cytometry - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)
    Flow Cytometry - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)
    Overlay histogram showing SHSY-5Y cells stained with ab52768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52768, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52768).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)

    Immunohistochemical staining of MAP1LC3A in paraffin embedded human breast carcinoma using ab52768 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52768).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)
    Anti-MAP1LC3A antibody [EP1983Y] - BSA and Azide free (ab239849)

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