Immunofluorescence analysis of MAP1LC3A expression in SH-SY5Y (human neuroblastoma cell line from bone marrow) cells.
Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

Immunofluorescence analysis of MAP1LC3A expression in C6 (rat glial tumor cell line) cells.
Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

Flow cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells of MAP1LC3A with ab244210.
Fixing / permeabilization of cells: Absolute methanol / 0.1% Tween-20 in PBS.
Primary antibody: ab244210 at 2 μg per ~10⁶ cells for 15 minutes at room temperature.
Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1/1000) with incubation for 15 minutes in dark at room temperature.
Non-specific Control IgG, clone X63 was used under same conditions as negative control (black dashed).

Anti-MAP1LC3A antibody [BS405] (ab244210) at 10 µg/ml (overnight at 4degC) + SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg

Secondary
Anti-mouse-HRP, 2 hrs at RT at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa

SDS-PAGE: 4-20% Bis-Tris denatured.