Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9107] to LYPD3 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LYPD3 antibody [EPR9107] - BSA and Azide free
    See all LYPD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9107] to LYPD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249029 is the carrier-free version of ab151709 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249029 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9107

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    Western blot - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (purified)

    Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Human esophagus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 dilution

    Predicted band size: 36 kDa



    This data was developed using ab151709, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    This data was developed using ab151709, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling LYPD3 with Purified ab151709 at 1:1000 dilution (1.01 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    This data was developed using ab151709, the same antibody clone in a different buffer formulation.ab151709 (purified) at 1:50 dilution (2ug) immunoprecipitating LYPD3 in HaCaT (Human skin keratinocyte) whole cell lysate.
    Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10ug
    Lane 2 (+): ab151709 & HaCaT (Human skin keratinocyte) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151709 in HaCaT (Human skin keratinocyte) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Western blot - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    Western blot - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (unpurified)

    Lane 1 : BxPC3 cell lysate
    Lane 2 : HACAT cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 36 kDa



    This data was developed using ab151709, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    This data was developed using ab151709, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling LYPD3 with unpurified ab151709 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
    Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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