Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
![Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)](https://www.abcam.com/ps/products/249/ab249029/Images/ab249029-410325-anti-lypd3-antibody-epr9107-bsa-and-azide-free-western-blot.jpg "Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9107] to LYPD3 - BSA and Azide free
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-LYPD3 antibody [EPR9107] - BSA and Azide free
See all LYPD3 primary antibodies -
Description
Rabbit monoclonal [EPR9107] to LYPD3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249029 is the carrier-free version of ab151709 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249029 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9107 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (purified)
Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Human esophagus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 dilution
Predicted band size: 36 kDaThis data was developed using ab151709, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)](https://www.abcam.com/ps/products/249/ab249029/Images/ab249029-4-anti-lypd3-antibody-epr9107-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)This data was developed using ab151709, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling LYPD3 with Purified ab151709 at 1:1000 dilution (1.01 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. -
![Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)](https://www.abcam.com/ps/products/249/ab249029/Images/ab249029-3-anti-lypd3-antibody-epr9107-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)This data was developed using ab151709, the same antibody clone in a different buffer formulation.ab151709 (purified) at 1:50 dilution (2ug) immunoprecipitating LYPD3 in HaCaT (Human skin keratinocyte) whole cell lysate.
Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10ug
Lane 2 (+): ab151709 & HaCaT (Human skin keratinocyte) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151709 in HaCaT (Human skin keratinocyte) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)](https://www.abcam.com/ps/products/249/ab249029/Images/ab249029-402304-anti-lypd3-antibody-epr9107-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (unpurified)
Lane 1 : BxPC3 cell lysate
Lane 2 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDaThis data was developed using ab151709, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)](https://www.abcam.com/ps/products/249/ab249029/Images/ab249029-1-anti-lypd3-antibody-epr9107-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)This data was developed using ab151709, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling LYPD3 with unpurified ab151709 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)](https://www.abcam.com/ps/products/249/ab249029/Images/ab249029-6-benefits-of-recombinant-antibodies.png)
Anti-LYPD3 antibody [EPR9107] - BSA and Azide free (ab249029)
