Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

Key features and details

  • Mouse monoclonal [OTI1D9] to LRRC25/MAPA
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-LRRC25/MAPA antibody [OTI1D9]

  • Description

    Mouse monoclonal [OTI1D9] to LRRC25/MAPA

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human LRRC25/MAPA aa 1 to the C-terminus. Produced in HEK-293T cell (NP_660299).
    Database link: Q8N386

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: pCMV6-ENTRY LRRC25/MAPA transfected HEK-293T cells. IHC-P: Human lung carcinoma, endometrium adenocarcinoma and lymphoma tissue. ICC/IF: pCMV6-ENTRY LRRC25/MAPA transfected COS-7 cells Flow: HEK-293T cells transfected with overexpressing plasmid.

  • General notes

    Clone OTI1D9 (formerly 1D9).

     This product was previously labelled as LRRC25

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)
    Western blot - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)
    All lanes : Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY LRRC25/MAPA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

    Paraffin-embedded human lung carcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)
    Immunocytochemistry/ Immunofluorescence - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

    COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with pCMV6-ENTRY LRRC25/MAPA labeling LRRC25/MAPA using ab236395 at 1/100 dilution in ICC/IF.

  • Flow Cytometry - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)
    Flow Cytometry - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

    Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

    Paraffin-embedded human lymphoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

    Paraffin-embedded human endometrium adenocarcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.

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