Antibodies, Proteins, Kits and Reagents for Life Science

Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

Key features and details

Mouse monoclonal [OTI1D9] to LRRC25/MAPA
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human, African green monkey
Isotype: IgG1

Product name

Anti-LRRC25/MAPA antibody [OTI1D9]
Description

Mouse monoclonal [OTI1D9] to LRRC25/MAPA
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human LRRC25/MAPA aa 1 to the C-terminus. Produced in HEK-293T cell (NP_660299).
Database link: Q8N386

Run BLAST with Run BLAST with
Positive control
- WB: pCMV6-ENTRY LRRC25/MAPA transfected HEK-293T cells. IHC-P: Human lung carcinoma, endometrium adenocarcinoma and lymphoma tissue. ICC/IF: pCMV6-ENTRY LRRC25/MAPA transfected COS-7 cells Flow: HEK-293T cells transfected with overexpressing plasmid.
General notes

Clone OTI1D9 (formerly 1D9).
This product was previously labelled as LRRC25

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI1D9
Isotype

IgG1
Research areas

Western blot - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

All lanes : Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395) at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY LRRC25/MAPA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

Paraffin-embedded human lung carcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with pCMV6-ENTRY LRRC25/MAPA labeling LRRC25/MAPA using ab236395 at 1/100 dilution in ICC/IF.
Flow Cytometry - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

Paraffin-embedded human lymphoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.

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