Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5997] to LSP1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LSP1 antibody [EPR5997] - BSA and Azide free
    See all LSP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5997] to LSP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon, Mouse spleen, Rat spleen and Human tonsil tissue. ICC/IF: DaudiFlow Cyt: Daudi

  • General notes

    Ab240063 is the carrier-free version of ab133506. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240063 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Immunocytochemistry/ Immunofluorescence - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (9.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Flow Cytometry - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling LSP1 with ab133506 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133506).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab133506 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133506).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    OI-RD Scanning - Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133506).

  • Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)
    Anti-LSP1 antibody [EPR5997] - BSA and Azide free (ab240063)

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