Anti-LIME antibody [EPR14713] (ab188871)
![Anti-LIME antibody [EPR14713] (ab188871)](https://www.abcam.com/ps/products/188/ab188871/Images/ab188871-225644-anti-lime-antibody-epr14713-immunohistochemistry.gif "Anti-LIME antibody [EPR14713] (ab188871)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14713] to LIME
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-LIME antibody [EPR14713]
See all LIME primary antibodies -
Description
Rabbit monoclonal [EPR14713] to LIME -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HuT-78 and Human thymus lysates; Human tonsil and mammary gland infiltrating duct carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14713 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] (ab188871)
Immunohistochemical analysis of paraffin-embedded Human mammary gland infiltrating duct carcinoma tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] (ab188871)](https://www.abcam.com/ps/products/188/ab188871/Images/ab188871-225643-anti-lime-antibody-epr14713-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] (ab188871)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-LIME antibody [EPR14713] (ab188871)](https://www.abcam.com/ps/products/188/ab188871/Images/ab188871-225642-anti-lime-antibody-epr14713-western-blot.jpg)
Western blot - Anti-LIME antibody [EPR14713] (ab188871)Anti-LIME antibody [EPR14713] (ab188871) at 1/1000 dilution + Human thymus lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution
Predicted band size: 31 kDa
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![Western blot - Anti-LIME antibody [EPR14713] (ab188871)](https://www.abcam.com/ps/products/188/ab188871/Images/ab188871-225641-anti-lime-antibody-epr14713-western-blot.jpg)
Western blot - Anti-LIME antibody [EPR14713] (ab188871)Anti-LIME antibody [EPR14713] (ab188871) at 1/10000 dilution + HuT-78 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 31 kDa
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![Anti-LIME antibody [EPR14713] (ab188871)](https://www.abcam.com/ps/products/188/ab188871/Images/ab188871-5-benefits-of-recombinant-antibodies.png)
Anti-LIME antibody [EPR14713] (ab188871)