Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21757-33] to LGALS3BP
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LGALS3BP antibody [EPR21757-33]
    See all LGALS3BP primary antibodies

  • Description

    Rabbit monoclonal [EPR21757-33] to LGALS3BP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human LGALS3BP aa 1-400. The exact sequence is proprietary.
    Database link: Q08380

  • Positive control

    • WB: Human fetal brain, fetal kidney and heart lysate; HepG2, C6, RAW 264.7, PC-12, NIH/3T3, MCF7, A549, HeLa, 4T1 and Hepa1-6 whole cell lysate; Rat brain, liver and spleen lysate. Mouse heart and kidney lysate. Flow: MCF7 and HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21757-33

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 10 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat liver lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : Mouse kidney lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 7 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 9 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lanes 3 and 4: 136 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lanes 6,7,8 and 9: 103 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Lanes 1-4 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/5000 dilution
    Lanes 5-6 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 5 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
    Lane 6 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 97, 70 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 15 seconds; Lanes 5 and 6: 81 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).

  • Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
    Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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