Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21757-33] to LGALS3BP
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-LGALS3BP antibody [EPR21757-33]
See all LGALS3BP primary antibodies
Description

Rabbit monoclonal [EPR21757-33] to LGALS3BP
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment within Human LGALS3BP aa 1-400. The exact sequence is proprietary.
Database link: Q08380
Positive control
- WB: Human fetal brain, fetal kidney and heart lysate; HepG2, C6, RAW 264.7, PC-12, NIH/3T3, MCF7, A549, HeLa, 4T1 and Hepa1-6 whole cell lysate; Rat brain, liver and spleen lysate. Mouse heart and kidney lysate. Flow: MCF7 and HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21757-33
Isotype

IgG
Research areas

Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

Predicted band size: 65 kDa
Observed band size: 97, 70 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

All lanes : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse kidney lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 7 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 9 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 65 kDa
Observed band size: 97, 70 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 15 seconds; Lanes 3 and 4: 136 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lanes 6,7,8 and 9: 103 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Lanes 1-4 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/5000 dilution
Lanes 5-6 : Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution

Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
Lane 6 : Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 65 kDa
Observed band size: 97, 70 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 15 seconds; Lanes 5 and 6: 81 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
Flow Cytometry - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Anti-LGALS3BP antibody [EPR21757-33] (ab217572)

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