Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LGALS3BP antibody [OTI3D6] (ab236509)

Key features and details

  • Mouse monoclonal [OTI3D6] to LGALS3BP
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

Overview

  • Product name

    Anti-LGALS3BP antibody [OTI3D6]
    See all LGALS3BP primary antibodies

  • Description

    Mouse monoclonal [OTI3D6] to LGALS3BP

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Recombinant fragment
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human LGALS3BP aa 1-300. (NP_005558) produced in E.coli.
    Database link: Q08380

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T transfected with r pCMV6-ENTRY LGALS3BP cDNA, whole cell lysate. IHC-P: Human lung carcinoma, endometrium adenocarcinoma, prostate and bladder carcinoma tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY LGALS3BP cDNA. Flow: HEK-293T cells.

  • General notes

    Clone OTI3D6 (formerly 3D6).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from cell culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    OTI3D6

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    Western blot - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    All lanes : Anti-LGALS3BP antibody [OTI3D6] (ab236509) at 1/500 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA, whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY LGALS3BP cDNA, whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)

    Paraffin-embedded human lung carcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)

    Paraffin-embedded human endometrium adenocarcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)

    Paraffin-embedded human prostate tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (ab236509)

    Paraffin-embedded human bladder carcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [OTI3D6] (ab236509)

    COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with pCMV6-ENTRY LGALS3BP cDNA stained for LGALS3BP (green) using ab236509 at 1/100 dilution in ICC/IF.

  • Flow Cytometry - Anti-LGALS3BP antibody [OTI3D6] (ab236509)
    Flow Cytometry - Anti-LGALS3BP antibody [OTI3D6] (ab236509)

    Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with empty vector control (blue) or vector expressing LGALS3BP (red), labeling LGALS3BP with ab236509 at 1/100 dilution.

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