Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Cycle Assay Kit (Fluorometric - Red)
See all Cell Cycle kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).
Visit our FAQs page for tips and troubleshooting. -
Notes
Store ab112117 desiccated.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 100X RNase A 1 x 250µl Assay Buffer 1 x 50ml Nuclear Red CCS1 1 x 250µl -
Research areas
Images
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Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)DNA profile in growing Jurkat cells.
Jurkat cells were dye-loaded with Red Fluorescence Probe and RNase A for 30 minutes. The fluorescence intensity of Red Fluorescence Probe was measured with a flow cytometer, using the FL2 channel.
