Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)
Key features and details
- Detection method: Fluorescent
- Sample type: Adherent cells, Suspension cells
Overview
-
Product name
Cell Cycle Assay Kit (Fluorometric - Green)
See all Cell Cycle kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Green CCS1 can be monitored with a flow cytometer at Ex/Em = 490 nm/520 nm (FL1 channel).
Visit our FAQs page for tips and troubleshooting.
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 50ml Nuclear Green CCS1 1 x 250µl -
Research areas
Images
-
Flow cytometry - ab112116 Cell Cycle Assay kit (Fluorometric-Green)
Histogram of HeLa cells (black: untreated cells, red : apoptotic cells) stained with 0.1x Nuclear Green CCS1
-
Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)DNA profile in growing and camptothecin treated Jurkat cells.
Jurkat cells were treated without (red) or with 20 µM camptothecin (blue) in a 37°C, 5% CO2 incubator for ~8 hours, then dye loaded with 200X Green Fluorescence Probe for 60 minutes. The fluorescence intensity of 200X Green Fluorescence Probe was measured with a flow cytometer using the FL1 channel.
